rabbit polyclonal antibodies against aurora-a  (Novus Biologicals)

Journal: Oncology Letters

Article Title: Receptor tyrosine kinase expression in high-grade gliomas before and after chemoradiotherapy

doi: 10.3892/ol.2019.11017

Figure Lengend Snippet: Significantly changed receptor tyrosine kinases between initial and recurrent gliomas.

Article Snippet: 5% skim milk was used to block the membrane at room temperature for 1 h. Primary rabbit polyclonal antibodies against IGF1R (ProteinTech Group, Inc.; cat. no. 10297-1-AP; dilution, 1:200) were used at 4°C overnight.

Techniques:

Journal: Oncology Letters

Article Title: Receptor tyrosine kinase expression in high-grade gliomas before and after chemoradiotherapy

doi: 10.3892/ol.2019.11017

Figure Lengend Snippet: IGFR was activated by TMZ. (A) Western blot analysis revealed that IGF1R expression was increased in glioma cells after treatment with TMZ. Expression levels was normalized by the level of GAPDH and expressed as fold-changes of those in parental cells. The data are presented as mean ± SD. ***P<0.001 as indicated. (B) Data analysis of 135 initial and 47 recurrent high-grade glioma cases revealed that IGFR signaling was highly activated in recurrent gliomas. TMZ, temozolomide; IGF1R, insulin-like growth factor 1 receptor.

Article Snippet: 5% skim milk was used to block the membrane at room temperature for 1 h. Primary rabbit polyclonal antibodies against IGF1R (ProteinTech Group, Inc.; cat. no. 10297-1-AP; dilution, 1:200) were used at 4°C overnight.

Techniques: Western Blot, Expressing

Journal: Oncotarget

Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas

doi:

Figure Lengend Snippet: A. The protein expression of Ki-67, AURKA and NDC80 was significantly increased with increasing OD grades, and semiquantitative analysis was performed in 5 NBTs, 57 OIIs and 29 OIIIs; B. Kaplan–Meier survival analysis showed that high expression of these three proteins indicated a poor survival. The columns represent the same samples; 511 and 1278 refer to the sample ID in the CGGA dataset.

Article Snippet: Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.

Techniques: Expressing

Journal: Oncotarget

Article Title: Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas

doi:

Figure Lengend Snippet: A. The three proteins (Ki-67, AURKA and NDC80) showed similar expression levels and significantly increased with increasing tumor grades; B. Western blotting showed that suppression of the expression of AURKA and NDC80 with siRNAs significantly decreased their protein expression; C. and D. MTT assay and cell immunofluorescence showed that suppression of AURKA and NDC80 expression suppressed the malignant proliferation of glioma cells and Ki-67 expression. Parental cells are non-transfected H4 and LN229 cells; Negative Control refers to transfected H4 and LN229 cells with a scrambled siRNA sequence that will not lead to specific degradation of any known cellular mRNA.

Article Snippet: Primary rabbit polyclonal antibodies against AURKA (Proteintech Group, Inc., Catalog No.: 10297–1-AP, 1:200) and NDC80 (Proteintech Group, Inc., Catalog No.: 18932–1-AP, 1:500) were used.

Techniques: Expressing, Western Blot, MTT Assay, Immunofluorescence, Transfection, Negative Control, Sequencing